Trimming

Input Reads

../20171018_oly_pacbio.correctedReads.fasta.gz
Parameterspreset = N/A
defaultQV = 20
isNonRandom = false
removeDuplicateReads = true
finalTrim = true
removeSpurReads = true
removeChimericReads = true
checkForSubReads = true
FASTA1665075 reads (11661226162 bp)
0 reads (0 bp) were short and not loaded
FASTQ0 reads (0 bp)
0 reads (0 bp) were short and not loaded
1665075 reads (11661226162 bp) loaded, 0 reads (0 bp) skipped, 0 warnings

Final Store

trimming/20171018_oly_pacbio.gkpStore
readsLoaded1665075 reads (11661226162 bp)
readsSkipped0 reads (0 bp) (read was too short)
warnings0 warnings (invalid base or quality value)

Read Length Histogram

k-Mer Counts

Histogram for k=22 with 11626259587 mers, 8324680157 distinct mers and 7725326153 single-copy mers. Largest count is 2538138.

Overlaps

Overlaps not computed.

Trimming

PARAMETERS
1000reads trimmed below this many bases are deleted
0.0750use overlaps at or below this fraction error
1break region if overlap is less than this long, for 'largest covered' algorithm
1break region if overlap coverage is less than this many read, for 'largest covered' algorithm
INPUT READS
readsbases
166507511661226162reads processed
00reads not processed, previously deleted
00reads not processed, in a library where trimming isn't allowed
OUTPUT READS
readsbases
7124813458657537trimmed reads output
1078559293896reads with no change, kept as is
8678505755379456reads with no overlaps, deleted
73959499189010reads with short trimmed length, deleted
TRIMMING DETAILS
readsbases
6391251149862364bases trimmed from the 5' end of a read
686805738843899bases trimmed from the 3' end of a read

Image 'trimming/3-overlapbasedtrimming/20171018_oly_pacbio.1.trimReads.inputDeletedReads' not found.

Image 'trimming/3-overlapbasedtrimming/20171018_oly_pacbio.1.trimReads.inputNoTrimReads' not found.

Image 'trimming/3-overlapbasedtrimming/20171018_oly_pacbio.1.trimReads.inputReads' not found.

Image 'trimming/3-overlapbasedtrimming/20171018_oly_pacbio.1.trimReads.outputDeletedReads' not found.

Image 'trimming/3-overlapbasedtrimming/20171018_oly_pacbio.1.trimReads.outputNoOvlReads' not found.

Image 'trimming/3-overlapbasedtrimming/20171018_oly_pacbio.1.trimReads.outputTrimmedReads' not found.

Image 'trimming/3-overlapbasedtrimming/20171018_oly_pacbio.1.trimReads.outputUnchangedReads' not found.

Image 'trimming/3-overlapbasedtrimming/20171018_oly_pacbio.1.trimReads.trim3' not found.

Image 'trimming/3-overlapbasedtrimming/20171018_oly_pacbio.1.trimReads.trim5' not found.

Splitting

PARAMETERS
1000reads trimmed below this many bases are deleted
0.0750use overlaps at or below this fraction error
INPUT READS
readsbases
7232665406657696reads processed
9418096254568466reads not processed, previously deleted
00reads not processed, in a library where trimming isn't allowed
PROCESSED
readsbases
00no overlaps
321124048944no coverage after adjusting for trimming done already
00processed for chimera
00processed for spur
7200555382608752processed for subreads
READS WITH SIGNALS
readssignals
00number of 5' spur signal
00number of 3' spur signal
00number of chimera signal
12891310number of subread signal
SIGNALS
readsbases
00size of 5' spur signal
00size of 3' spur signal
00size of chimera signal
1310418184size of subread signal
TRIMMING
readsbases
6552190728trimmed from the 5' end of the read
6372255145trimmed from the 3' end of the read