Command: Programfile g GAP_VALUE k KMER_SIZE LD LOAD_SKG GS GENOME_SIZE TrimN TRIM_READS_WITH_N f INPUT_FILE1 f INPUT_FILE2 i1 INWARD_PAIR_END1 i2 INWARD_PAIR_END2 o1 OUTWARD_PAIR_END1 o2 OUTWARD_PAIR_END2 Parameters: k: kmer size, support 15~127. g: number of skipped intermediate k-mers, support 1-25. f: single end reads. Multiple inputs shall be independently imported with this parameter. i1 & i2 (or p1 & p2): inward paired-end reads. o1 & o2 (or l1 & l2): outward paired-end reads. GS: genome size estimation in bp (used for memory pre-allocation), suggest a large value if possible.(e.g. ~ 2x genome size) LD: load a saved k-mer graph. BC: 1: build contigs.0: don't build. KmerTable: 1 if you want to output the kmer table. NodeCovTh: coverage threshold for spurious k-mers, support 0-16. (default 1) EdgeCovTh: coverage threshold for spurious links, support 0-16. (default 0) PathCovTh: coverage threshold for spurious paths in the breadth-first search, support 0-100. TrimLen: trim long sequences to this length. TrimN: throw away reads with more than this number of Ns. TrimQual: trim off tails with quality scores lower than this. QualBase: lowest quality score value (in ASCII value) in the current fastq scoring system, default: '!'. For error correction: Denoise: use 1 to call the error correction module. (default 0) H: hybrid mode. 0 (Default): reads will be trimmed at the ends to ensure denoising accuracy (*MUST* set 0 for the last round). 1: reads will not be trimmed at the ends; CovTh: coverage threshold for an error. A k-mer with coverage < this value will be checked. Setting 0 will allow the program to choose a value based on the coverage histogram. CorrTh: coverage threshold for a correct k-mer candidate. A k-mer with coverage >= this value will be considered a candidate for correction. Setting 0 will allow the program to choose a value based on the coverage histogram. For scaffolding: ExpCov: expected average k-mer coverage in a unique contig. Used for scaffolding. Scaffold: 1: scaffolding with paired reads. 0: single end assembly. LinkCovTh: coverage threshold for spurious paired-end links, support 0-100. (default 5) Iter_Scaffold: 1: iterative scaffolding using the already built scaffolds (/super contigs). 0: one round scaffolding. For mate pair scaffolding: InsertSize: estimated insert size of the current pair. i1_mp & i2_mp: inward mate paired reads (large insert sizes >10k, for shorter libraries omit "_mp"). o1_mp & o2_mp : outward paired-end reads (large insert sizes >10k, for shorter libraries omit "_mp"). Scanning the dataset. 1/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/AD002_S9_L001_R1_001_val_1_val_1.fastq Reading: 50000000 Reading: 100000000 Reading: 150000000 2/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/AD002_S9_L001_R2_001_val_2_val_2.fastq Reading: 200000000 Reading: 250000000 Reading: 300000000 3/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/AD002_S9_L002_R1_001_val_1_val_1.fastq Reading: 350000000 Reading: 400000000 Reading: 450000000 Reading: 500000000 4/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/AD002_S9_L002_R2_001_val_2_val_2.fastq Reading: 550000000 Reading: 600000000 Reading: 650000000 Reading: 700000000 Reading: 750000000 5/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR005_S4_L001_R1_001_val_1_val_1.fastq 6/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR005_S4_L001_R2_001_val_2_val_2.fastq 7/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR005_S4_L002_R1_001_val_1_val_1.fastq 8/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR005_S4_L002_R2_001_val_2_val_2.fastq 9/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR006_S3_L001_R1_001_val_1_val_1.fastq 10/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR006_S3_L001_R2_001_val_2_val_2.fastq 11/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR006_S3_L002_R1_001_val_1_val_1.fastq 12/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR006_S3_L002_R2_001_val_2_val_2.fastq 13/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR012_S1_L001_R1_001_val_1_val_1.fastq 14/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR012_S1_L001_R2_001_val_2_val_2.fastq 15/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR012_S1_L002_R1_001_val_1_val_1.fastq 16/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR012_S1_L002_R2_001_val_2_val_2.fastq 17/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR013_AD013_S2_L001_R1_001_val_1_val_1.fastq Reading: 800000000 Reading: 850000000 Reading: 900000000 Reading: 950000000 Reading: 1000000000 Reading: 1050000000 18/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR013_AD013_S2_L001_R2_001_val_2_val_2.fastq Reading: 1100000000 Reading: 1150000000 Reading: 1200000000 Reading: 1250000000 Reading: 1300000000 Reading: 1350000000 19/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR013_AD013_S2_L002_R1_001_val_1_val_1.fastq Reading: 1400000000 Reading: 1450000000 Reading: 1500000000 Reading: 1550000000 Reading: 1600000000 Reading: 1650000000 Reading: 1700000000 Reading: 1750000000 20/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR013_AD013_S2_L002_R2_001_val_2_val_2.fastq Reading: 1800000000 Reading: 1850000000 Reading: 1900000000 Reading: 1950000000 Reading: 2000000000 Reading: 2050000000 Reading: 2100000000 21/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR014_AD014_S5_L001_R1_001_val_1_val_1.fastq Reading: 2150000000 Reading: 2200000000 Reading: 2250000000 Reading: 2300000000 Reading: 2350000000 Reading: 2400000000 Reading: 2450000000 Reading: 2500000000 Reading: 2550000000 Reading: 2600000000 Reading: 2650000000 Reading: 2700000000 Reading: 2750000000 Reading: 2800000000 Reading: 2850000000 22/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR014_AD014_S5_L001_R2_001_val_2_val_2.fastq Reading: 2900000000 Reading: 2950000000 Reading: 3000000000 Reading: 3050000000 Reading: 3100000000 Reading: 3150000000 Reading: 3200000000 Reading: 3250000000 Reading: 3300000000 Reading: 3350000000 Reading: 3400000000 Reading: 3450000000 Reading: 3500000000 Reading: 3550000000 Reading: 3600000000 23/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR014_AD014_S5_L002_R1_001_val_1_val_1.fastq Reading: 3650000000 Reading: 3700000000 Reading: 3750000000 Reading: 3800000000 Reading: 3850000000 Reading: 3900000000 Reading: 3950000000 Reading: 4000000000 Reading: 4050000000 Reading: 4100000000 Reading: 4150000000 Reading: 4200000000 Reading: 4250000000 Reading: 4300000000 Reading: 4350000000 Reading: 4400000000 Reading: 4450000000 Reading: 4500000000 24/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR014_AD014_S5_L002_R2_001_val_2_val_2.fastq Reading: 4550000000 Reading: 4600000000 Reading: 4650000000 Reading: 4700000000 Reading: 4750000000 Reading: 4800000000 Reading: 4850000000 Reading: 4900000000 Reading: 4950000000 Reading: 5000000000 Reading: 5050000000 Reading: 5100000000 Reading: 5150000000 Reading: 5200000000 Reading: 5250000000 Reading: 5300000000 Reading: 5350000000 25/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR015_AD015_S6_L001_R1_001_val_1_val_1.fastq Reading: 5400000000 Reading: 5450000000 Reading: 5500000000 Reading: 5550000000 Reading: 5600000000 26/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR015_AD015_S6_L001_R2_001_val_2_val_2.fastq Reading: 5650000000 Reading: 5700000000 Reading: 5750000000 Reading: 5800000000 Reading: 5850000000 27/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR015_AD015_S6_L002_R1_001_val_1_val_1.fastq Reading: 5900000000 Reading: 5950000000 Reading: 6000000000 Reading: 6050000000 Reading: 6100000000 Reading: 6150000000 28/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR015_AD015_S6_L002_R2_001_val_2_val_2.fastq Reading: 6200000000 Reading: 6250000000 Reading: 6300000000 Reading: 6350000000 Reading: 6400000000 Reading: 6450000000 29/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR019_S7_L001_R1_001_val_1_val_1.fastq 30/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR019_S7_L001_R2_001_val_2_val_2.fastq 31/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR019_S7_L002_R1_001_val_1_val_1.fastq 32/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR019_S7_L002_R2_001_val_2_val_2.fastq 33/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR021_S8_L001_R1_001_val_1_val_1.fastq 34/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR021_S8_L001_R2_001_val_2_val_2.fastq 35/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR021_S8_L002_R1_001_val_1_val_1.fastq 36/36 files. Processing file: /gscratch/scrubbed/samwhite/20180129_trimmed_again/NR021_S8_L002_R2_001_val_2_val_2.fastq Scan finished. Error! K-mer size too large!