FastQCFastQC Report
Tue 27 Mar 2018
160103_I137_FCH3V5YBBXX_L4_WHPANwalDDAADWAAPEI-101_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename160103_I137_FCH3V5YBBXX_L4_WHPANwalDDAADWAAPEI-101_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences63723614
Sequences flagged as poor quality0
Sequence length49
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG76200.010.55249343
ACGGGGG44850.09.6354525
CGGGGGG87150.08.6098686
GACGGGG49500.08.0787884
GATCGGA113550.07.27080641
AGATCGG122000.07.137295243
ATCGGAA125300.06.84636942
CGGGGCG39250.06.1898091
GCGGGGG80300.06.1581575
GCCGGCG21600.05.8726854
CGGCGCG14400.05.8229176
CCGGGGG39400.05.7296965
CGGGGGC36750.05.5578236
CGTGGCG37200.05.3172041