FastQCFastQC Report
Tue 27 Mar 2018
160103_I137_FCH3V5YBBXX_L5_WHPANwalDDACDTAAPEI-102_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename160103_I137_FCH3V5YBBXX_L5_WHPANwalDDACDTAAPEI-102_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences41762399
Sequences flagged as poor quality0
Sequence length49
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG47500.022.26947430
TCACGCG54700.019.6526527
CACGCGA55750.019.16681728
CGGAAGA62150.018.0925181
ACGCGAA61550.017.46547529
GCGAAGT65650.016.5384631
GTCACGC66400.016.48117426
CGTATGC69350.015.99711643
GCACACG72850.015.4056268
GAACTCC75250.014.91428418
GAAGAGC80450.013.8701053
AATCTCG76550.013.64990138
AGTCACG82650.013.42286825
CGAAGTA86600.012.95958332
TCGTATG90200.012.65687342
CTCGTAT89200.012.48542641
TCTCGTA82950.012.3893940
ACGTCTG92650.012.34538612
CGTCTGA96200.011.95686113
ATCTCGT89500.011.89106239
Produced by FastQC (version 0.11.5)