FastQCFastQC Report
Tue 27 Mar 2018
160103_I137_FCH3V5YBBXX_L6_WHPANwalDDABDLAAPEI-100_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename160103_I137_FCH3V5YBBXX_L6_WHPANwalDDABDLAAPEI-100_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences73632218
Sequences flagged as poor quality0
Sequence length49
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGC66050.010.51400532
CGGAAGA78700.08.8513341
GAACTCC84950.08.55444318
TCGTAAG87950.08.33598731
GCACACG86400.08.2864588
TCACTCG92000.07.758695627
CGTATGC91050.07.745194443
CTCGTAA95800.07.563152330
GAAGAGC99200.07.3256053
CACTCGT105150.06.95197328
TCTCGTA101900.06.751717640
CATCTCG108500.06.47972438
CTCGTAT119900.06.419516641
ACTCGTA118250.06.163635729
CGTCTGA124900.06.11088913
TCGTATG124150.06.095852442
ACGTCTG128000.05.962890612
ATCTCGT119050.05.85132339
CACGTCT130150.05.7983111
GTCACTC136400.05.769061626