FastQCFastQC Report
Tue 27 Mar 2018
160103_I137_FCH3V5YBBXX_L6_WHPANwalDDACDTAAPEI-102_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename160103_I137_FCH3V5YBBXX_L6_WHPANwalDDACDTAAPEI-102_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences42108576
Sequences flagged as poor quality0
Sequence length49
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCG15300.012.6470583
TCGCGGG18400.010.6331525
TTCGCGG17350.08.798274
CGCGGGG22200.08.7162166
ACTTCGC38350.08.6336372
CGGGGGG59100.06.839255313
TCGGAAG51350.06.61538443
CGCGGGA17350.06.5677236
CTTCGCC26750.06.42990643
TACTTCG60150.06.1479631
GGGCGCT15500.05.82580628
CGGGGCG24250.05.7628868
GCGGGGG55000.05.707272512
GGTCGCC22750.05.670329643
GATCGGA64450.05.637703443
CGCGTGC16050.05.626168325
TCGCGTG23450.05.592755
AGATCGG72700.05.441540743
TCGCGCG14255.456968E-125.4315795
GCGGGGC26600.05.41541347