FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-2to4kb-5_S18_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-2to4kb-5_S18_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266864
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3207612.019605491935966No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2980.11166736614904971Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT1302.9071089E-838.1548968
TCGGAAG1354.053618E-836.7417533
GAGCGTC1354.053618E-836.7417539
CGATCAC700.002275219735.4295469
CGGAAGA1457.600829E-834.2078364
GATCGGA1501.0143049E-733.1028441
CGGATTT800.004354144431.0339161
CGAATCC1003.456888E-431.0339161
GAAGAGC1753.9542283E-728.3436376
CGGACTG900.00774211827.5857031
ATCGGAA2001.263832E-624.8112562
GGAAGAG2001.2685377E-624.8006845
AAGAGCG2101.9396975E-623.6196987
CGAATGT1400.0024636422.1670841
GTATGGA1753.6003714E-421.2667922
CGTACAT2250.001941120616.5514221
GTGGTCG1500.00679950815.783781145
CGTGTGT3351.0737021E-414.8221681
GGTCGCC1403.5560897E-49.26073140-44
TGGTCGC1403.5560897E-49.26073140-44
Produced by FastQC (version 0.11.5)