FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-5to7kb-3_S11_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-5to7kb-3_S11_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991000
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13172413.2920282542886No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGGG2451.8224651E-551.136925145
GAGGCGG2700.001809194237.121616145
CAGGCGG3651.9127414E-434.324783145
CGCGCCG2950.002793697633.97572145
TGAGGGG2950.002793697633.97572145
CGGACGG3150.003852522731.81853145
ACCGCCG3200.004161244331.321363145
GTACAGG4556.960729E-427.535267145
TTTCCGT5657.875268E-526.609303145
CTCACTT5600.002334500422.372402145
TCCTCTT6600.006047263718.982647145
CGTATCG2200.00162806916.999821
CGTAACG2350.002543649415.901768
CGTTCAG3156.153743E-515.830521
CGGAGTG2800.0080996413.3570011
CGTGTAA4509.0037014E-512.4665351
CGTTGTT8051.0477379E-912.3891031
CGTAAAA5907.3768733E-611.62134551
CGTCTAT4550.001377891210.9595911
CGTAACA4750.0019720110.4981341
Produced by FastQC (version 0.11.5)