FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-5to7kb-6_S23_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-5to7kb-6_S23_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337457885
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5004702414.830598490830937No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7404080.2194075269570305Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC4277900.022.8890539
GATCGGA4266750.022.872251
TCGGAAG4356550.022.4974983
CGGAAGA4426700.022.2427644
AGAGCGT4454100.022.0376668
AAGAGCG4512100.021.776977
ATCGGAA4585000.021.4138032
GAAGAGC4636750.021.2429646
GGAAGAG5316400.018.6525345
CGCGCGG314000.011.987319145
CGCGGGG386700.011.804688145
GGCGCGG413050.011.4394145
GCGCGGG377650.011.168637145
CGGGGGG681250.011.011143145
CCGCGCG314650.010.774784145
AGATCGG3451800.010.7508951
CGGTGGG679550.010.488719145
TCGCCGG529650.010.28191145
TCGCGGG313400.09.965968145
GCGCCGG409500.09.843638145
Produced by FastQC (version 0.11.5)