FastQCFastQC Report
Thu 29 Mar 2018
Geoduck-NMP-gDNA-2_S5_L002_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-2_S5_L002_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298518471
Sequences flagged as poor quality0
Sequence length20-151
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGGG936300.07.010371145
GTCCCTT1759700.06.77982145
GCCCGGG293200.06.617483145
CGTTCGG331650.06.472652145
GCGCGGG208051.8189894E-126.349525145
GTCGAGG425400.06.3077555145
CGGGGGG408400.06.267075145
CTCGACG298600.06.221307145
GGAACGG471800.06.2123995145
CGTGAGG339050.06.209625145
CGCGATG422600.06.1541767145
CGTACGG276100.06.1302247145
CTCACGG424900.06.1208644145
CTAGCGG325950.06.0792384145
GCAACGG478450.05.953488145
CGGGCGG459700.05.9269104145
GGCTTCG292700.05.923597145
GCACAGG802900.05.9128294145
GCGTAGG339550.05.835747145
GAGACGG737900.05.8182817145
Produced by FastQC (version 0.11.5)