FastQCFastQC Report
Thu 29 Mar 2018
Geoduck-NMP-gDNA-2to4kb-3_S10_L003_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-2to4kb-3_S10_L003_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284280784
Sequences flagged as poor quality0
Sequence length20-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG441500.07.451771145
CGATCGG307850.07.3472323145
GGCGCGG244200.07.241408145
CGCGCGG162105.820766E-116.8498483145
GCGCGGG215650.06.674501145
GCGGGGG570000.06.565484145
CGAAGGG309450.06.511872145
GAGAGGG1042350.06.430937145
GGAGAGG976800.06.399384145
GGGGGGG3313250.06.3177695145
GTCACGG700500.06.222974145
GCGTAGG380400.06.1621823145
GCTACGG408450.06.141736145
CGTGTGG780050.06.062833145
CGCGGGG231401.8189894E-126.0424876145
GTGTGGG1760000.05.935007145
CGTAGGG312200.05.9276123145
GAGTCGG409350.05.927307145
GGAAGGG963800.05.845664145
GAGGAGG931000.05.830751145
Produced by FastQC (version 0.11.5)