FastQCFastQC Report
Mon 2 Apr 2018
Geoduck-NMP-gDNA-5to7kb-4_S15_L004_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-5to7kb-4_S15_L004_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651379
Sequences flagged as poor quality0
Sequence length20-151
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGACG3000.00550355255.632088145
GTCTTTT7850.006740560828.347563145
CGTGGTG2353.3069227E-822.7035981
CGTCTAC1500.00882706417.7844851
CGTGTTG2452.7071008E-517.4215351
CGTCTCA2553.815771E-516.7383381
CGTGGTA2050.002870058615.6156451
CGTGCCA2050.002870058615.6156451
CGTACTT2700.001114023213.8323771
CGTTTTG4552.2978347E-612.8986371
CGTTACA3100.003148719712.0475541
CGTTTTA6307.6122487E-610.1625631
CGTTTGT4750.0071276418.9858451
CGTATAT7304.1681624E-48.0395611
CGTTTTC6150.0065705117.8078221
CAGCCCC3000.0075111767.6932316135-139
CCCCGCC7350.00110607435.2334905135-139
CATTAAA14750.00402202745.0605574
Produced by FastQC (version 0.11.5)