FastQCFastQC Report
Tue 3 Apr 2018
Geoduck-NMP-gDNA-5to7kb-5_S19_L005_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-5to7kb-5_S19_L005_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235822630
Sequences flagged as poor quality0
Sequence length20-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG235550.07.029661145
GTCCCTT1280700.06.859634145
GGCTCTT647500.06.535262145
GCCTCTT889800.06.4614887145
GCTTCGG248250.06.299479145
CCTTCGG266250.06.2191052145
GATGCGG347800.06.0833564145
GATCGGG301300.05.953611145
GCGTAGG295150.05.9218283145
CGATCGG234904.645699E-95.482643145
GAACGGG321100.05.44325145
TTCACGG587700.05.400182145
CGACAGG457700.05.3261075145
CTCTCTT1504300.05.228494145
TTCTCTT2520550.05.2007327145
GGATCGG363550.05.18722145
CTCTGCG302301.1277734E-105.173158145
CCTACGG215256.797454E-75.1284137145
GTCGTGG762400.05.0677056145
GGGGGGG2015100.05.0672383145
Produced by FastQC (version 0.11.5)