FastQCFastQC Report
Tue 3 Apr 2018
Geoduck-NMP-gDNA-5to7kb-6_S23_L006_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-5to7kb-6_S23_L006_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237876509
Sequences flagged as poor quality0
Sequence length20-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTT1314950.07.1540966145
GCCTCTT897400.06.5582147145
GCGTAGG289400.06.0848947145
CTTCGCG169401.7107959E-76.0183473145
CTCGTGG355800.05.9912834145
CGTAGGG232107.8216544E-115.989824145
CGCGCTT268203.6379788E-125.8747325145
CTCGAGG279351.8189894E-125.806138145
GTCGCTT793150.05.7258334145
GATCGCG219404.5529305E-95.7028923145
CGGGGGG285453.6379788E-125.682062145
CGTGTGG626750.05.471487145
CCGTAGG213308.487768E-85.4314666145
CGATGGG307705.456968E-125.4217935145
CCTCCTT835850.05.322441145
TTCTCTT2565450.05.3107145
CCTTCGG273109.349606E-105.260269145
CTCTGCG302509.276846E-115.2086062145
CTCTCTT1514350.05.2022433145
GGCTCTT651950.05.188915145
Produced by FastQC (version 0.11.5)