FastQCFastQC Report
Wed 4 Apr 2018
Geoduck-NMP-gDNA-8to10kb-8_S32_L008_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-8to10kb-8_S32_L008_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285982479
Sequences flagged as poor quality0
Sequence length20-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTT1635750.07.5681925145
CGTTCGG310750.06.98913145
GCCTCTT1106050.06.6370664145
CGTACGG288900.06.615602145
CGATCGG263950.06.41811145
GTAGCGG519650.06.185645145
CGAAGGG288000.06.1838026145
GGCTCTT809850.06.0609136145
GCGGGGG458000.05.8801775145
CTTCGGG299300.05.8052044145
GCGCGGG180755.9158992E-85.7676272145
CTCGAGG327950.05.6954107145
CGCGCTT331500.05.634419145
GGCGCGG200951.610897E-85.620172145
ACGCGGG227351.4533725E-95.540734145
GAGGAGG884200.05.5021415145
CTGCGTG602600.05.4783363145
CAACGGG491900.05.474937145
CGATGGG378700.05.390969145
CTCGTGG437200.05.3651145
Produced by FastQC (version 0.11.5)