SUMMARISING RUN PARAMETERS ========================== Input filename: Geoduck-NMP-gDNA-2to4kb-2_S6_L002_R1_001.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4_dev Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/samwhite/illumina_geoduck_hiseq/20180328_trim_galore_illumina_hiseq_geoduck/20180328_fastqc_trimmed_hiseq_geoduck --threads 28 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.14 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC Geoduck-NMP-gDNA-2to4kb-2_S6_L002_R1_001.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.17 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 46,064 Reads with adapters: 12,711 (27.6%) Reads written (passing filters): 46,064 (100.0%) Total basepairs processed: 6,334,661 bp Quality-trimmed: 208,056 bp (3.3%) Total written (filtered): 6,110,027 bp (96.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 12711 times. No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 35.7% C: 24.3% G: 13.0% T: 26.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 10159 11516.0 0 10159 2 1792 2879.0 0 1792 3 557 719.8 0 557 4 163 179.9 0 163 5 22 45.0 0 22 6 6 11.2 0 6 7 3 2.8 0 3 10 1 0.0 1 1 12 1 0.0 1 1 30 1 0.0 1 0 1 31 1 0.0 1 1 34 1 0.0 1 0 1 44 1 0.0 1 1 51 1 0.0 1 1 60 1 0.0 1 1 73 1 0.0 1 0 1 RUN STATISTICS FOR INPUT FILE: Geoduck-NMP-gDNA-2to4kb-2_S6_L002_R1_001.fastq.gz ============================================= 46064 sequences processed in total