SUMMARISING RUN PARAMETERS ========================== Input filename: Geoduck-NMP-gDNA-4_S13_L004_R1_001.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4_dev Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/samwhite/illumina_geoduck_hiseq/20180328_trim_galore_illumina_hiseq_geoduck/20180328_fastqc_trimmed_hiseq_geoduck --threads 28 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.14 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC Geoduck-NMP-gDNA-4_S13_L004_R1_001.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.44 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 54,441 Reads with adapters: 13,028 (23.9%) Reads written (passing filters): 54,441 (100.0%) Total basepairs processed: 7,139,942 bp Quality-trimmed: 243,519 bp (3.4%) Total written (filtered): 6,878,887 bp (96.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 13028 times. No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 34.4% C: 23.5% G: 14.0% T: 28.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 10104 13610.2 0 10104 2 2184 3402.6 0 2184 3 511 850.6 0 511 4 178 212.7 0 178 5 32 53.2 0 32 6 3 13.3 0 3 7 2 3.3 0 2 8 2 0.8 0 2 9 1 0.2 0 1 14 1 0.0 1 0 1 17 1 0.0 1 0 1 36 1 0.0 1 1 42 1 0.0 1 1 43 1 0.0 1 0 1 48 1 0.0 1 0 1 55 1 0.0 1 0 1 56 1 0.0 1 0 1 78 1 0.0 1 1 96 1 0.0 1 0 1 117 1 0.0 1 1 RUN STATISTICS FOR INPUT FILE: Geoduck-NMP-gDNA-4_S13_L004_R1_001.fastq.gz ============================================= 54441 sequences processed in total