SUMMARISING RUN PARAMETERS ========================== Input filename: Geoduck-NMP-gDNA-5_S17_L005_R1_001.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4_dev Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/samwhite/illumina_geoduck_hiseq/20180328_trim_galore_illumina_hiseq_geoduck/20180328_fastqc_trimmed_hiseq_geoduck --threads 28 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.14 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC Geoduck-NMP-gDNA-5_S17_L005_R1_001.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.35 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 25,880 Reads with adapters: 5,985 (23.1%) Reads written (passing filters): 25,880 (100.0%) Total basepairs processed: 3,517,457 bp Quality-trimmed: 92,838 bp (2.6%) Total written (filtered): 3,415,401 bp (97.1%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 5985 times. No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 33.5% C: 25.0% G: 13.7% T: 27.7% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 4640 6470.0 0 4640 2 988 1617.5 0 988 3 241 404.4 0 241 4 82 101.1 0 82 5 10 25.3 0 10 6 1 6.3 0 1 7 1 1.6 0 1 10 2 0.0 1 0 2 11 1 0.0 1 1 34 1 0.0 1 1 43 1 0.0 1 0 1 53 1 0.0 1 1 62 1 0.0 1 1 67 1 0.0 1 1 70 1 0.0 1 0 1 73 3 0.0 1 0 3 75 1 0.0 1 0 1 76 4 0.0 1 4 77 1 0.0 1 1 80 1 0.0 1 0 1 94 1 0.0 1 0 1 128 1 0.0 1 0 1 151 1 0.0 1 0 1 RUN STATISTICS FOR INPUT FILE: Geoduck-NMP-gDNA-5_S17_L005_R1_001.fastq.gz ============================================= 25880 sequences processed in total