SUMMARISING RUN PARAMETERS ========================== Input filename: Geoduck-NMP-gDNA-5to7kb-1_S3_L001_R1_001.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4_dev Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/samwhite/illumina_geoduck_hiseq/20180328_trim_galore_illumina_hiseq_geoduck/20180328_fastqc_trimmed_hiseq_geoduck --threads 28 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.14 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC Geoduck-NMP-gDNA-5to7kb-1_S3_L001_R1_001.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.93 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 63,365 Reads with adapters: 15,099 (23.8%) Reads written (passing filters): 63,365 (100.0%) Total basepairs processed: 8,424,415 bp Quality-trimmed: 237,122 bp (2.8%) Total written (filtered): 8,166,503 bp (96.9%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 15099 times. No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 34.9% C: 24.3% G: 13.6% T: 27.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 11998 15841.2 0 11998 2 2190 3960.3 0 2190 3 621 990.1 0 621 4 205 247.5 0 205 5 45 61.9 0 45 6 9 15.5 0 9 7 3 3.9 0 3 8 1 1.0 0 1 10 1 0.1 1 0 1 12 2 0.0 1 1 1 15 3 0.0 1 1 2 17 1 0.0 1 0 1 22 1 0.0 1 0 1 23 1 0.0 1 1 26 1 0.0 1 0 1 34 1 0.0 1 0 1 36 1 0.0 1 1 41 1 0.0 1 0 1 47 1 0.0 1 1 54 2 0.0 1 1 1 55 1 0.0 1 1 63 1 0.0 1 1 69 1 0.0 1 1 70 1 0.0 1 1 71 1 0.0 1 1 89 1 0.0 1 0 1 98 1 0.0 1 0 1 102 2 0.0 1 1 1 122 1 0.0 1 0 1 147 1 0.0 1 0 1 RUN STATISTICS FOR INPUT FILE: Geoduck-NMP-gDNA-5to7kb-1_S3_L001_R1_001.fastq.gz ============================================= 63365 sequences processed in total