SUMMARISING RUN PARAMETERS ========================== Input filename: Geoduck-NMP-gDNA-5to7kb-2_S7_L002_R1_001.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4_dev Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/samwhite/illumina_geoduck_hiseq/20180328_trim_galore_illumina_hiseq_geoduck/20180328_fastqc_trimmed_hiseq_geoduck --threads 28 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.14 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC Geoduck-NMP-gDNA-5to7kb-2_S7_L002_R1_001.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.03 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 68,454 Reads with adapters: 16,462 (24.0%) Reads written (passing filters): 68,454 (100.0%) Total basepairs processed: 9,072,717 bp Quality-trimmed: 256,864 bp (2.8%) Total written (filtered): 8,793,704 bp (96.9%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 16462 times. No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 34.6% C: 24.3% G: 13.5% T: 27.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 13052 17113.5 0 13052 2 2407 4278.4 0 2407 3 685 1069.6 0 685 4 238 267.4 0 238 5 46 66.8 0 46 6 11 16.7 0 11 7 2 4.2 0 2 8 1 1.0 0 1 11 2 0.0 1 1 1 15 1 0.0 1 0 1 24 1 0.0 1 1 25 1 0.0 1 0 1 34 2 0.0 1 2 36 1 0.0 1 0 1 38 1 0.0 1 1 53 2 0.0 1 1 1 57 1 0.0 1 1 59 1 0.0 1 0 1 60 1 0.0 1 0 1 62 1 0.0 1 1 66 1 0.0 1 1 68 1 0.0 1 0 1 74 1 0.0 1 0 1 81 1 0.0 1 0 1 97 1 0.0 1 1 RUN STATISTICS FOR INPUT FILE: Geoduck-NMP-gDNA-5to7kb-2_S7_L002_R1_001.fastq.gz ============================================= 68454 sequences processed in total