SUMMARISING RUN PARAMETERS ========================== Input filename: Geoduck-NMP-gDNA-6_S21_L006_R1_001.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4_dev Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/samwhite/illumina_geoduck_hiseq/20180328_trim_galore_illumina_hiseq_geoduck/20180328_fastqc_trimmed_hiseq_geoduck --threads 28 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.14 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC Geoduck-NMP-gDNA-6_S21_L006_R1_001.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.40 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 29,238 Reads with adapters: 6,900 (23.6%) Reads written (passing filters): 29,238 (100.0%) Total basepairs processed: 3,979,508 bp Quality-trimmed: 101,141 bp (2.5%) Total written (filtered): 3,868,128 bp (97.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 6900 times. No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 32.3% C: 24.0% G: 14.8% T: 28.7% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 5416 7309.5 0 5416 2 1049 1827.4 0 1049 3 295 456.8 0 295 4 96 114.2 0 96 5 22 28.6 0 22 6 2 7.1 0 2 7 1 1.8 0 1 10 1 0.0 1 0 1 28 1 0.0 1 1 30 1 0.0 1 1 41 1 0.0 1 1 53 1 0.0 1 0 1 62 1 0.0 1 0 1 66 1 0.0 1 1 69 1 0.0 1 1 70 1 0.0 1 1 74 1 0.0 1 0 1 76 3 0.0 1 3 77 1 0.0 1 1 79 1 0.0 1 0 1 81 1 0.0 1 1 82 1 0.0 1 0 1 134 1 0.0 1 0 1 143 1 0.0 1 1 RUN STATISTICS FOR INPUT FILE: Geoduck-NMP-gDNA-6_S21_L006_R1_001.fastq.gz ============================================= 29238 sequences processed in total