SUMMARISING RUN PARAMETERS ========================== Input filename: Geoduck-NMP-gDNA-8_S29_L008_R1_001.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4_dev Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/samwhite/illumina_geoduck_hiseq/20180328_trim_galore_illumina_hiseq_geoduck/20180328_fastqc_trimmed_hiseq_geoduck --threads 28 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.14 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC Geoduck-NMP-gDNA-8_S29_L008_R1_001.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.56 s (53 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 10,428 Reads with adapters: 2,537 (24.3%) Reads written (passing filters): 10,428 (100.0%) Total basepairs processed: 1,373,621 bp Quality-trimmed: 73,308 bp (5.3%) Total written (filtered): 1,295,710 bp (94.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 2537 times. No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 31.5% C: 26.6% G: 14.4% T: 27.2% none/other: 0.2% Overview of removed sequences length count expect max.err error counts 1 1910 2607.0 0 1910 2 450 651.8 0 450 3 111 162.9 0 111 4 37 40.7 0 37 5 11 10.2 0 11 6 1 2.5 0 1 11 1 0.0 1 0 1 15 1 0.0 1 1 41 2 0.0 1 2 61 1 0.0 1 1 65 1 0.0 1 1 70 1 0.0 1 1 74 2 0.0 1 1 1 75 2 0.0 1 1 1 76 1 0.0 1 1 77 1 0.0 1 1 82 1 0.0 1 1 113 1 0.0 1 0 1 150 1 0.0 1 0 1 151 1 0.0 1 0 1 RUN STATISTICS FOR INPUT FILE: Geoduck-NMP-gDNA-8_S29_L008_R1_001.fastq.gz ============================================= 10428 sequences processed in total