SUMMARISING RUN PARAMETERS ========================== Input filename: Geoduck-NMP-gDNA-8to10kb-2_S8_L002_R1_001.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4_dev Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/samwhite/illumina_geoduck_hiseq/20180328_trim_galore_illumina_hiseq_geoduck/20180328_fastqc_trimmed_hiseq_geoduck --threads 28 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.14 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC Geoduck-NMP-gDNA-8to10kb-2_S8_L002_R1_001.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.18 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 21,702 Reads with adapters: 5,593 (25.8%) Reads written (passing filters): 21,702 (100.0%) Total basepairs processed: 2,994,474 bp Quality-trimmed: 110,023 bp (3.7%) Total written (filtered): 2,876,687 bp (96.1%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 5593 times. No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 35.3% C: 23.9% G: 13.5% T: 27.2% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 4384 5425.5 0 4384 2 900 1356.4 0 900 3 217 339.1 0 217 4 67 84.8 0 67 5 10 21.2 0 10 6 1 5.3 0 1 8 1 0.3 0 1 10 2 0.0 1 1 1 12 1 0.0 1 0 1 18 1 0.0 1 1 32 1 0.0 1 0 1 33 1 0.0 1 1 40 1 0.0 1 0 1 60 1 0.0 1 1 64 1 0.0 1 1 66 1 0.0 1 0 1 70 1 0.0 1 0 1 73 1 0.0 1 0 1 109 1 0.0 1 0 1 RUN STATISTICS FOR INPUT FILE: Geoduck-NMP-gDNA-8to10kb-2_S8_L002_R1_001.fastq.gz ============================================= 21702 sequences processed in total