SUMMARISING RUN PARAMETERS ========================== Input filename: Geoduck-NMP-gDNA-8to10kb-5_S20_L005_R1_001.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4_dev Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/samwhite/illumina_geoduck_hiseq/20180328_trim_galore_illumina_hiseq_geoduck/20180328_fastqc_trimmed_hiseq_geoduck --threads 28 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.14 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC Geoduck-NMP-gDNA-8to10kb-5_S20_L005_R1_001.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.66 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 87,562 Reads with adapters: 20,789 (23.7%) Reads written (passing filters): 87,562 (100.0%) Total basepairs processed: 11,436,175 bp Quality-trimmed: 304,214 bp (2.7%) Total written (filtered): 11,089,885 bp (97.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 20789 times. No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 34.3% C: 22.8% G: 13.7% T: 28.6% none/other: 0.6% Overview of removed sequences length count expect max.err error counts 1 16267 21890.5 0 16267 2 3095 5472.6 0 3095 3 873 1368.2 0 873 4 298 342.0 0 298 5 56 85.5 0 56 6 7 21.4 0 7 7 6 5.3 0 6 8 2 1.3 0 2 9 1 0.3 0 0 1 10 1 0.1 1 0 1 16 3 0.0 1 3 17 1 0.0 1 0 1 18 1 0.0 1 0 1 21 1 0.0 1 1 25 1 0.0 1 1 30 2 0.0 1 1 1 31 2 0.0 1 2 34 1 0.0 1 0 1 36 1 0.0 1 0 1 38 1 0.0 1 1 39 2 0.0 1 2 40 2 0.0 1 1 1 41 1 0.0 1 1 42 1 0.0 1 0 1 43 1 0.0 1 0 1 48 2 0.0 1 2 49 1 0.0 1 1 59 1 0.0 1 1 66 2 0.0 1 1 1 68 2 0.0 1 2 69 1 0.0 1 1 70 1 0.0 1 0 1 71 1 0.0 1 0 1 72 1 0.0 1 1 73 2 0.0 1 1 1 74 4 0.0 1 0 4 75 21 0.0 1 3 18 76 27 0.0 1 9 18 77 17 0.0 1 5 12 78 19 0.0 1 9 10 79 5 0.0 1 3 2 80 6 0.0 1 5 1 81 1 0.0 1 1 82 2 0.0 1 0 2 83 2 0.0 1 0 2 85 2 0.0 1 2 86 1 0.0 1 1 87 2 0.0 1 1 1 88 1 0.0 1 0 1 90 1 0.0 1 0 1 96 1 0.0 1 0 1 102 1 0.0 1 0 1 108 1 0.0 1 1 109 1 0.0 1 1 118 1 0.0 1 0 1 122 1 0.0 1 0 1 126 1 0.0 1 0 1 131 1 0.0 1 1 146 1 0.0 1 0 1 148 1 0.0 1 1 149 3 0.0 1 1 2 150 19 0.0 1 3 16 151 5 0.0 1 0 5 RUN STATISTICS FOR INPUT FILE: Geoduck-NMP-gDNA-8to10kb-5_S20_L005_R1_001.fastq.gz ============================================= 87562 sequences processed in total