Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/1_ATCACG_L001_R1_001_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/oly_genome/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 1000000 Number of alignments with a unique best hit from the different alignments: 402696 Mapping efficiency: 40.3% Sequences with no alignments under any condition: 120858 Sequences did not map uniquely: 476446 Sequences which were discarded because genomic sequence could not be extracted: 778 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 184982 ((converted) top strand) CT/GA: 185009 ((converted) bottom strand) GA/CT: 15907 (complementary to (converted) top strand) GA/GA: 16020 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2271556 Total methylated C's in CpG context: 89270 Total methylated C's in CHG context: 6460 Total methylated C's in CHH context: 118528 Total methylated C's in Unknown context: 31 Total unmethylated C's in CpG context: 220987 Total unmethylated C's in CHG context: 414387 Total unmethylated C's in CHH context: 1421924 Total unmethylated C's in Unknown context: 389 C methylated in CpG context: 28.8% C methylated in CHG context: 1.5% C methylated in CHH context: 7.7% C methylated in Unknown context (CN or CHN): 7.4% Bismark completed in 0d 0h 22m 37s