Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/2_CGATGT_L001_R1_001_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/oly_genome/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 1000000 Number of alignments with a unique best hit from the different alignments: 399343 Mapping efficiency: 39.9% Sequences with no alignments under any condition: 122371 Sequences did not map uniquely: 478286 Sequences which were discarded because genomic sequence could not be extracted: 802 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 182474 ((converted) top strand) CT/GA: 181356 ((converted) bottom strand) GA/CT: 17309 (complementary to (converted) top strand) GA/GA: 17402 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2256185 Total methylated C's in CpG context: 86136 Total methylated C's in CHG context: 9408 Total methylated C's in CHH context: 129242 Total methylated C's in Unknown context: 32 Total unmethylated C's in CpG context: 220939 Total unmethylated C's in CHG context: 405831 Total unmethylated C's in CHH context: 1404629 Total unmethylated C's in Unknown context: 408 C methylated in CpG context: 28.1% C methylated in CHG context: 2.3% C methylated in CHH context: 8.4% C methylated in Unknown context (CN or CHN): 7.3% Bismark completed in 0d 0h 44m 14s