Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/5_ACAGTG_L001_R1_001_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/oly_genome/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 1000000 Number of alignments with a unique best hit from the different alignments: 399336 Mapping efficiency: 39.9% Sequences with no alignments under any condition: 122548 Sequences did not map uniquely: 478116 Sequences which were discarded because genomic sequence could not be extracted: 797 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 182865 ((converted) top strand) CT/GA: 183372 ((converted) bottom strand) GA/CT: 16131 (complementary to (converted) top strand) GA/GA: 16171 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2260603 Total methylated C's in CpG context: 86479 Total methylated C's in CHG context: 6442 Total methylated C's in CHH context: 121234 Total methylated C's in Unknown context: 33 Total unmethylated C's in CpG context: 224327 Total unmethylated C's in CHG context: 413986 Total unmethylated C's in CHH context: 1408135 Total unmethylated C's in Unknown context: 359 C methylated in CpG context: 27.8% C methylated in CHG context: 1.5% C methylated in CHH context: 7.9% C methylated in Unknown context (CN or CHN): 8.4% Bismark completed in 0d 1h 49m 10s