Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_10_s456_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/oly_genome/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 1000000 Number of alignments with a unique best hit from the different alignments: 349287 Mapping efficiency: 34.9% Sequences with no alignments under any condition: 227005 Sequences did not map uniquely: 423708 Sequences which were discarded because genomic sequence could not be extracted: 834 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 88481 ((converted) top strand) CT/GA: 87841 ((converted) bottom strand) GA/CT: 86795 (complementary to (converted) top strand) GA/GA: 85336 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2181648 Total methylated C's in CpG context: 342902 Total methylated C's in CHG context: 9337 Total methylated C's in CHH context: 101372 Total methylated C's in Unknown context: 81 Total unmethylated C's in CpG context: 59857 Total unmethylated C's in CHG context: 471881 Total unmethylated C's in CHH context: 1196299 Total unmethylated C's in Unknown context: 373 C methylated in CpG context: 85.1% C methylated in CHG context: 1.9% C methylated in CHH context: 7.8% C methylated in Unknown context (CN or CHN): 17.8% Bismark completed in 0d 3h 13m 42s