Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_18_s456_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/oly_genome/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 1000000 Number of alignments with a unique best hit from the different alignments: 348941 Mapping efficiency: 34.9% Sequences with no alignments under any condition: 243194 Sequences did not map uniquely: 407865 Sequences which were discarded because genomic sequence could not be extracted: 643 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 75345 ((converted) top strand) CT/GA: 75330 ((converted) bottom strand) GA/CT: 99079 (complementary to (converted) top strand) GA/GA: 98544 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2096162 Total methylated C's in CpG context: 272848 Total methylated C's in CHG context: 7950 Total methylated C's in CHH context: 99835 Total methylated C's in Unknown context: 55 Total unmethylated C's in CpG context: 53734 Total unmethylated C's in CHG context: 434377 Total unmethylated C's in CHH context: 1227418 Total unmethylated C's in Unknown context: 350 C methylated in CpG context: 83.5% C methylated in CHG context: 1.8% C methylated in CHH context: 7.5% C methylated in Unknown context (CN or CHN): 13.6% Bismark completed in 0d 5h 46m 34s