Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_4_s456_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/oly_genome/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 1000000 Number of alignments with a unique best hit from the different alignments: 328404 Mapping efficiency: 32.8% Sequences with no alignments under any condition: 265459 Sequences did not map uniquely: 406137 Sequences which were discarded because genomic sequence could not be extracted: 674 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 72131 ((converted) top strand) CT/GA: 71250 ((converted) bottom strand) GA/CT: 92342 (complementary to (converted) top strand) GA/GA: 92007 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2003787 Total methylated C's in CpG context: 280951 Total methylated C's in CHG context: 8854 Total methylated C's in CHH context: 100565 Total methylated C's in Unknown context: 45 Total unmethylated C's in CpG context: 44679 Total unmethylated C's in CHG context: 404574 Total unmethylated C's in CHH context: 1164164 Total unmethylated C's in Unknown context: 347 C methylated in CpG context: 86.3% C methylated in CHG context: 2.1% C methylated in CHH context: 8.0% C methylated in Unknown context (CN or CHN): 11.5% Bismark completed in 0d 7h 3m 15s