Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_9_s456_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/oly_genome/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 1000000 Number of alignments with a unique best hit from the different alignments: 346612 Mapping efficiency: 34.7% Sequences with no alignments under any condition: 242736 Sequences did not map uniquely: 410652 Sequences which were discarded because genomic sequence could not be extracted: 674 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 76661 ((converted) top strand) CT/GA: 76331 ((converted) bottom strand) GA/CT: 97008 (complementary to (converted) top strand) GA/GA: 95938 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2052482 Total methylated C's in CpG context: 232563 Total methylated C's in CHG context: 7170 Total methylated C's in CHH context: 97165 Total methylated C's in Unknown context: 62 Total unmethylated C's in CpG context: 54861 Total unmethylated C's in CHG context: 402828 Total unmethylated C's in CHH context: 1257895 Total unmethylated C's in Unknown context: 337 C methylated in CpG context: 80.9% C methylated in CHG context: 1.7% C methylated in CHH context: 7.2% C methylated in Unknown context (CN or CHN): 15.5% Bismark completed in 0d 8h 40m 21s