FastQCFastQC Report
Wed 16 May 2018
EPI-135WG_S42_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-135WG_S42_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176822437
Sequences flagged as poor quality0
Sequence length101
%GC27

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCAATAACACCCAATTTTTAAACCTAACTCATATAAAAATCAATCATC1859890.10518404969161238No Hit
ACCCAATAACACCCAATTTTTAATCATAACTCATATAAAAATCAATCATC1821680.10302312483115476No Hit
ACCACCCTCCAATAATACTTAAATTTCAATAAACACATCAATTATCTTCT1771960.10021126447883985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)