FastQCFastQC Report
Wed 16 May 2018
EPI-152_S3_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-152_S3_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30318886
Sequences flagged as poor quality0
Sequence length101
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG465590.15356434929700255TruSeq Adapter, Index 2 (100% over 49bp)
GGTAATATTTGGGTTTTAATGGATATGTTAGTTGTTTTTTATATTTTTTG308040.10160003899879434No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)