FastQCFastQC Report
Wed 16 May 2018
EPI-188_S21_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-188_S21_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24209277
Sequences flagged as poor quality0
Sequence length101
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG3018731.2469310834850624TruSeq Adapter, Index 8 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT575420.23768574336193518TruSeq Adapter, Index 8 (100% over 48bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT480830.1986139445634828TruSeq Adapter, Index 8 (100% over 43bp)
CCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT319980.13217247256082865TruSeq Adapter, Index 8 (100% over 43bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA319400.13193289498071337TruSeq Adapter, Index 8 (100% over 47bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)