FastQCFastQC Report
Wed 16 May 2018
EPI-205_S26_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-205_S26_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26556310
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG3901371.4690934094382841TruSeq Adapter, Index 1 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT943760.3553806985985628TruSeq Adapter, Index 1 (100% over 48bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT642280.24185589037031122TruSeq Adapter, Index 1 (100% over 43bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT625350.2354807576805663TruSeq Adapter, Index 1 (100% over 43bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT615630.23182061061947237TruSeq Adapter, Index 1 (100% over 43bp)
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT588760.22170248803391737TruSeq Adapter, Index 1 (100% over 43bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA527750.1987286637337793TruSeq Adapter, Index 1 (100% over 47bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT502630.18926951824255703TruSeq Adapter, Index 1 (100% over 43bp)
AGCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT482810.18180613195131404TruSeq Adapter, Index 1 (100% over 43bp)
CAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT473430.17827401472569043TruSeq Adapter, Index 1 (100% over 43bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC449390.1692215522412564TruSeq Adapter, Index 1 (100% over 44bp)
CACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT442920.1667852197839233TruSeq Adapter, Index 1 (100% over 43bp)
CCACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT417680.1572808872919468TruSeq Adapter, Index 1 (100% over 43bp)
CCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT384300.14471136991547395TruSeq Adapter, Index 1 (100% over 43bp)
CCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT383700.1444854349117027TruSeq Adapter, Index 1 (100% over 43bp)
CCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT381440.1436344130641644TruSeq Adapter, Index 1 (100% over 43bp)
ACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC357510.13462337199708843TruSeq Adapter, Index 1 (100% over 44bp)
ACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCG350770.1320853687880583TruSeq Adapter, Index 1 (100% over 45bp)
CACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT342390.1289298099020534TruSeq Adapter, Index 1 (100% over 43bp)
CCCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT336250.1266177416967945TruSeq Adapter, Index 1 (100% over 43bp)
ACCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT333950.1257516575156714TruSeq Adapter, Index 1 (100% over 43bp)
ACCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT323220.12171118653156254TruSeq Adapter, Index 1 (100% over 43bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT317240.1194593676606426TruSeq Adapter, Index 1 (100% over 48bp)
ACGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT313500.1180510394704686TruSeq Adapter, Index 1 (100% over 43bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA309570.11657116519576705TruSeq Adapter, Index 1 (100% over 47bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA304200.11454904691201452TruSeq Adapter, Index 1 (100% over 47bp)
CCCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT297360.11197338786902247TruSeq Adapter, Index 1 (100% over 43bp)
AGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC291220.10966131966376352TruSeq Adapter, Index 1 (100% over 44bp)
ACAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT284980.10731159562454272TruSeq Adapter, Index 1 (100% over 43bp)
AGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT277870.10463426582985362TruSeq Adapter, Index 1 (100% over 43bp)
ACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCG274490.10336149864194236TruSeq Adapter, Index 1 (100% over 45bp)
CCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT269200.10136950502535932TruSeq Adapter, Index 1 (100% over 43bp)
CCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC268100.10095529085177872TruSeq Adapter, Index 1 (100% over 44bp)
CAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC266740.10044317150989727TruSeq Adapter, Index 1 (100% over 44bp)
CCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC265590.10001012941933574TruSeq Adapter, Index 1 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)