FastQCFastQC Report
Wed 16 May 2018
EPI-206_S27_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-206_S27_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36712077
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG4948901.3480305132286576TruSeq Adapter, Index 2 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT1162320.3166042607722794TruSeq Adapter, Index 2 (100% over 48bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT875610.23850734459943523TruSeq Adapter, Index 2 (100% over 43bp)
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT781240.21280190712173547TruSeq Adapter, Index 2 (100% over 43bp)
CAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT726470.19788311078122878TruSeq Adapter, Index 2 (100% over 43bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT712880.19418133166369203TruSeq Adapter, Index 2 (100% over 43bp)
AGCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT680340.18531776341610964TruSeq Adapter, Index 2 (100% over 43bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTA651540.17747293349815102TruSeq Adapter, Index 2 (100% over 47bp)
CACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT640860.17456380907024138TruSeq Adapter, Index 2 (100% over 43bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC608700.1658037489951876TruSeq Adapter, Index 2 (100% over 44bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT604060.1645398597306276TruSeq Adapter, Index 2 (100% over 43bp)
CCACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT601810.1639269823932871TruSeq Adapter, Index 2 (100% over 43bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT599000.16316156669643073TruSeq Adapter, Index 2 (100% over 43bp)
ACAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT570660.1554420361452173TruSeq Adapter, Index 2 (100% over 43bp)
CACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT526310.14336154285141645TruSeq Adapter, Index 2 (100% over 43bp)
ACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC525170.14305101833383058TruSeq Adapter, Index 2 (100% over 44bp)
CCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT503130.1370475443271706TruSeq Adapter, Index 2 (100% over 43bp)
ACGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT477540.13007708607715113TruSeq Adapter, Index 2 (100% over 43bp)
CCCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT449290.12238207061943131TruSeq Adapter, Index 2 (100% over 43bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT448120.12206337440401424TruSeq Adapter, Index 2 (100% over 48bp)
AACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT442600.12055978200307219TruSeq Adapter, Index 2 (100% over 43bp)
ACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCG442260.12046716942765183TruSeq Adapter, Index 2 (100% over 45bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTA439210.1196363801481458TruSeq Adapter, Index 2 (100% over 47bp)
CCCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT426550.11618792366337649TruSeq Adapter, Index 2 (100% over 43bp)
AGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC425810.11598635511687339TruSeq Adapter, Index 2 (100% over 44bp)
CCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT415700.11323249294775668TruSeq Adapter, Index 2 (100% over 43bp)
ACCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT413300.11257875712126011TruSeq Adapter, Index 2 (100% over 43bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTA409330.11149736910826376TruSeq Adapter, Index 2 (100% over 47bp)
CAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC404520.11018717355599358TruSeq Adapter, Index 2 (100% over 44bp)
AGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT403840.1100019484051529TruSeq Adapter, Index 2 (100% over 43bp)
ATGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT399930.10893690378781892TruSeq Adapter, Index 2 (100% over 43bp)
CCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT398950.10866996165866617TruSeq Adapter, Index 2 (100% over 43bp)
ACCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT393180.10709827177579738TruSeq Adapter, Index 2 (100% over 43bp)
CCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC389830.10618576551797929TruSeq Adapter, Index 2 (100% over 44bp)
AACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT382290.10413194546306928TruSeq Adapter, Index 2 (100% over 43bp)
ACCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT376830.10264469645778962TruSeq Adapter, Index 2 (100% over 43bp)
ACTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT372060.10134539650262774TruSeq Adapter, Index 2 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)