FastQCFastQC Report
Wed 16 May 2018
EPI-208_S28_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-208_S28_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39075361
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4767741.2201397192466117Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC1046420.2677953506302859Illumina Single End PCR Primer 1 (100% over 49bp)
GGCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG789640.20208130642734176Illumina Single End PCR Primer 1 (100% over 44bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG614270.15720136277179883Illumina Single End PCR Primer 1 (100% over 48bp)
GGGCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG595510.15240038345391102Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG515870.1320192537696581Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG499540.12784014970456703Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG478660.12249662901386887Illumina Single End PCR Primer 1 (100% over 44bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC459220.11752162699149471Illumina Single End PCR Primer 1 (100% over 49bp)
GCGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG456320.11677947134000885Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG427600.10942957123287998Illumina Single End PCR Primer 1 (100% over 45bp)
GGGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG418340.10705979146296307Illumina Single End PCR Primer 1 (100% over 44bp)
TCTACATAAACCACAATCAAAAACTAAATATCACTAAACAAACCAAAAAA392770.10051602594279296No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)