FastQCFastQC Report
Wed 16 May 2018
EPI-43_S40_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-43_S40_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29360787
Sequences flagged as poor quality0
Sequence length101
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG2424220.8256658787790668TruSeq Adapter, Index 4 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT608470.20723899533074502TruSeq Adapter, Index 4 (100% over 48bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT422050.1437461468590743TruSeq Adapter, Index 4 (100% over 43bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT390130.13287450367049086TruSeq Adapter, Index 4 (100% over 43bp)
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT385260.13121582878551585TruSeq Adapter, Index 4 (100% over 43bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT376500.12823225753451364TruSeq Adapter, Index 4 (100% over 43bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTA348240.11860717493710232TruSeq Adapter, Index 4 (100% over 47bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT313490.10677166112747591TruSeq Adapter, Index 4 (100% over 43bp)
CAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT313130.10664904861031144TruSeq Adapter, Index 4 (100% over 43bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC309090.10527306369546566TruSeq Adapter, Index 4 (100% over 44bp)
AGCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT303520.10337597558267085TruSeq Adapter, Index 4 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph