Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/4_TGACCA_L001_R1_001_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 11408856 Number of alignments with a unique best hit from the different alignments: 4536160 Mapping efficiency: 39.8% Sequences with no alignments under any condition: 1477731 Sequences did not map uniquely: 5394965 Sequences which were discarded because genomic sequence could not be extracted: 8697 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2075415 ((converted) top strand) CT/GA: 2075298 ((converted) bottom strand) GA/CT: 187772 (complementary to (converted) top strand) GA/GA: 188978 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 25520936 Total methylated C's in CpG context: 1016597 Total methylated C's in CHG context: 68499 Total methylated C's in CHH context: 1376350 Total methylated C's in Unknown context: 409 Total unmethylated C's in CpG context: 2445956 Total unmethylated C's in CHG context: 4625068 Total unmethylated C's in CHH context: 15988466 Total unmethylated C's in Unknown context: 4191 C methylated in CpG context: 29.4% C methylated in CHG context: 1.5% C methylated in CHH context: 7.9% C methylated in Unknown context (CN or CHN): 8.9% Bismark completed in 0d 14h 30m 22s