Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/5_ACAGTG_L001_R1_001_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 12231319 Number of alignments with a unique best hit from the different alignments: 4798520 Mapping efficiency: 39.2% Sequences with no alignments under any condition: 1651146 Sequences did not map uniquely: 5781653 Sequences which were discarded because genomic sequence could not be extracted: 9631 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2187003 ((converted) top strand) CT/GA: 2186040 ((converted) bottom strand) GA/CT: 207926 (complementary to (converted) top strand) GA/GA: 207920 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 27207691 Total methylated C's in CpG context: 1043108 Total methylated C's in CHG context: 84105 Total methylated C's in CHH context: 1546253 Total methylated C's in Unknown context: 435 Total unmethylated C's in CpG context: 2688305 Total unmethylated C's in CHG context: 4955714 Total unmethylated C's in CHH context: 16890206 Total unmethylated C's in Unknown context: 4529 C methylated in CpG context: 28.0% C methylated in CHG context: 1.7% C methylated in CHH context: 8.4% C methylated in Unknown context (CN or CHN): 8.8% Bismark completed in 0d 18h 32m 9s