Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/7_CAGATC_L001_R1_001_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 10113854 Number of alignments with a unique best hit from the different alignments: 3984767 Mapping efficiency: 39.4% Sequences with no alignments under any condition: 1329633 Sequences did not map uniquely: 4799454 Sequences which were discarded because genomic sequence could not be extracted: 7873 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 1824319 ((converted) top strand) CT/GA: 1820642 ((converted) bottom strand) GA/CT: 165565 (complementary to (converted) top strand) GA/GA: 166368 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 22414088 Total methylated C's in CpG context: 854767 Total methylated C's in CHG context: 57383 Total methylated C's in CHH context: 1200345 Total methylated C's in Unknown context: 349 Total unmethylated C's in CpG context: 2163006 Total unmethylated C's in CHG context: 4071784 Total unmethylated C's in CHH context: 14066803 Total unmethylated C's in Unknown context: 3847 C methylated in CpG context: 28.3% C methylated in CHG context: 1.4% C methylated in CHH context: 7.9% C methylated in Unknown context (CN or CHN): 8.3% Bismark completed in 1d 1h 51m 27s