Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_11_s456_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 54458572 Number of alignments with a unique best hit from the different alignments: 16320566 Mapping efficiency: 30.0% Sequences with no alignments under any condition: 15053965 Sequences did not map uniquely: 23084041 Sequences which were discarded because genomic sequence could not be extracted: 40206 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3664176 ((converted) top strand) CT/GA: 3633017 ((converted) bottom strand) GA/CT: 4511175 (complementary to (converted) top strand) GA/GA: 4471992 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 104048121 Total methylated C's in CpG context: 18520646 Total methylated C's in CHG context: 492903 Total methylated C's in CHH context: 5003405 Total methylated C's in Unknown context: 3654 Total unmethylated C's in CpG context: 2984829 Total unmethylated C's in CHG context: 20797118 Total unmethylated C's in CHH context: 56249220 Total unmethylated C's in Unknown context: 21750 C methylated in CpG context: 86.1% C methylated in CHG context: 2.3% C methylated in CHH context: 8.2% C methylated in Unknown context (CN or CHN): 14.4% Bismark completed in 2d 20h 46m 16s