Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_12_s456_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 59459431 Number of alignments with a unique best hit from the different alignments: 20890554 Mapping efficiency: 35.1% Sequences with no alignments under any condition: 15643544 Sequences did not map uniquely: 22925333 Sequences which were discarded because genomic sequence could not be extracted: 37887 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 4663862 ((converted) top strand) CT/GA: 4630478 ((converted) bottom strand) GA/CT: 5799691 (complementary to (converted) top strand) GA/GA: 5758636 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 124396429 Total methylated C's in CpG context: 14492989 Total methylated C's in CHG context: 455769 Total methylated C's in CHH context: 6351378 Total methylated C's in Unknown context: 2695 Total unmethylated C's in CpG context: 3513867 Total unmethylated C's in CHG context: 25149482 Total unmethylated C's in CHH context: 74432944 Total unmethylated C's in Unknown context: 21222 C methylated in CpG context: 80.5% C methylated in CHG context: 1.8% C methylated in CHH context: 7.9% C methylated in Unknown context (CN or CHN): 11.3% Bismark completed in 3d 14h 28m 32s