Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_16_s456_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 74531056 Number of alignments with a unique best hit from the different alignments: 22243864 Mapping efficiency: 29.8% Sequences with no alignments under any condition: 20848664 Sequences did not map uniquely: 31438528 Sequences which were discarded because genomic sequence could not be extracted: 52465 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 5085546 ((converted) top strand) CT/GA: 5056511 ((converted) bottom strand) GA/CT: 6047871 (complementary to (converted) top strand) GA/GA: 6001471 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 139396921 Total methylated C's in CpG context: 23216932 Total methylated C's in CHG context: 629407 Total methylated C's in CHH context: 6853423 Total methylated C's in Unknown context: 4650 Total unmethylated C's in CpG context: 3820019 Total unmethylated C's in CHG context: 27364516 Total unmethylated C's in CHH context: 77512624 Total unmethylated C's in Unknown context: 27879 C methylated in CpG context: 85.9% C methylated in CHG context: 2.2% C methylated in CHH context: 8.1% C methylated in Unknown context (CN or CHN): 14.3% Bismark completed in 7d 2h 3m 26s