Bismark

Bismark Processing Report

/home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_17_s456_trimmed.fq.gz

Data processed at 15:02 on 2018-08-13


Alignment

Sequences analysed in total 42,070,546
Single-end alignments with a unique best hit 13,402,022
Sequences without alignments under any condition 11,125,453
Sequences that did not map uniquely 17,543,071
Genomic sequence context not extractable (edges of chromosomes) 28,967
Created with Highcharts 5.0.5: 31.8 %Unique AlignmentsUnique Alignments: 31.8 %: 26.4 %No AlignmentNo Alignment: 26.4 %: 41.7 %Multiple AlignmentsMultiple Alignments: 41.7 %: 0.1 %No Genomic SequenceNo Genomic Sequence: 0.1 %

Cytosine Methylation

Total C's analysed 83,239,424
Methylated C's in CpG context 13,500,223
Methylated C's in CHG context 381,439
Methylated C's in CHH context 4,279,479
Methylated C's in Unknown context 2,449
Unmethylated C's in CpG context 2,090,683
Unmethylated C's in CHG context 17,571,927
Unmethylated C's in CHH context 45,415,673
Unmethylated C's in Unknown context 16,276
Percentage methylation (CpG context) 86.6%
Percentage methylation (CHG context) 2.1%
Percentage methylation (CHH context) 8.6%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 3,040,693 original top strand
CTOT 3,666,380 complementary to original top strand
CTOB 3,643,625 complementary to original bottom strand
OB 3,022,357 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB01M2M3M4M

Deduplication

Alignments analysed 13,373,055
Unique alignments 8,163,478
Duplicates removed 5,209,577
Duplicated alignments were found at 1794464 different positions
Created with Highcharts 5.0.5: 61.0 %UniqueUnique: 61.0 %: 39.0 %DuplicateDuplicate: 39.0 %

Cytosine Methylation after Extraction

Total C's analysed 49,945,483
Methylated C's in CpG context 7,022,752
Methylated C's in CHG context 249,647
Methylated C's in CHH context 3,189,408
Unmethylated C's in CpG context 1,231,609
Unmethylated C's in CHG context 9,955,503
Unmethylated C's in CHH context 28,296,564
Percentage methylation (CpG context) 85.1%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 10.1%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

M-Bias Plot

Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 1Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls05101520253001020304050607080901000200k400k600k800k1 000k1 200k