Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_18_s456_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 55048106 Number of alignments with a unique best hit from the different alignments: 18869098 Mapping efficiency: 34.3% Sequences with no alignments under any condition: 14051321 Sequences did not map uniquely: 22127687 Sequences which were discarded because genomic sequence could not be extracted: 37148 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 4120413 ((converted) top strand) CT/GA: 4097029 ((converted) bottom strand) GA/CT: 5326285 (complementary to (converted) top strand) GA/GA: 5288223 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 113572211 Total methylated C's in CpG context: 14713606 Total methylated C's in CHG context: 443442 Total methylated C's in CHH context: 5621164 Total methylated C's in Unknown context: 2746 Total unmethylated C's in CpG context: 2907663 Total unmethylated C's in CHG context: 23410658 Total unmethylated C's in CHH context: 66475678 Total unmethylated C's in Unknown context: 19896 C methylated in CpG context: 83.5% C methylated in CHG context: 1.9% C methylated in CHH context: 7.8% C methylated in Unknown context (CN or CHN): 12.1% Bismark completed in 8d 6h 34m 2s