Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_1_s456_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 46792286 Number of alignments with a unique best hit from the different alignments: 15172526 Mapping efficiency: 32.4% Sequences with no alignments under any condition: 12808090 Sequences did not map uniquely: 18811670 Sequences which were discarded because genomic sequence could not be extracted: 31126 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3422666 ((converted) top strand) CT/GA: 3396694 ((converted) bottom strand) GA/CT: 4180880 (complementary to (converted) top strand) GA/GA: 4141160 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 92043982 Total methylated C's in CpG context: 12334507 Total methylated C's in CHG context: 420089 Total methylated C's in CHH context: 4799236 Total methylated C's in Unknown context: 2519 Total unmethylated C's in CpG context: 2543172 Total unmethylated C's in CHG context: 18362426 Total unmethylated C's in CHH context: 53584552 Total unmethylated C's in Unknown context: 16907 C methylated in CpG context: 82.9% C methylated in CHG context: 2.2% C methylated in CHH context: 8.2% C methylated in Unknown context (CN or CHN): 13.0% Bismark completed in 8d 20h 10m 27s