Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_3_s456_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 48376729 Number of alignments with a unique best hit from the different alignments: 15419326 Mapping efficiency: 31.9% Sequences with no alignments under any condition: 13562727 Sequences did not map uniquely: 19394676 Sequences which were discarded because genomic sequence could not be extracted: 32144 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3436184 ((converted) top strand) CT/GA: 3414515 ((converted) bottom strand) GA/CT: 4287404 (complementary to (converted) top strand) GA/GA: 4249079 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 92831596 Total methylated C's in CpG context: 12035424 Total methylated C's in CHG context: 405969 Total methylated C's in CHH context: 4939429 Total methylated C's in Unknown context: 2598 Total unmethylated C's in CpG context: 2449981 Total unmethylated C's in CHG context: 18062610 Total unmethylated C's in CHH context: 54938183 Total unmethylated C's in Unknown context: 17632 C methylated in CpG context: 83.1% C methylated in CHG context: 2.2% C methylated in CHH context: 8.2% C methylated in Unknown context (CN or CHN): 12.8% Bismark completed in 9d 23h 37m 31s