Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_4_s456_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 44905078 Number of alignments with a unique best hit from the different alignments: 14481141 Mapping efficiency: 32.2% Sequences with no alignments under any condition: 12422799 Sequences did not map uniquely: 18001138 Sequences which were discarded because genomic sequence could not be extracted: 29863 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3213378 ((converted) top strand) CT/GA: 3191749 ((converted) bottom strand) GA/CT: 4034139 (complementary to (converted) top strand) GA/GA: 4012012 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 88412183 Total methylated C's in CpG context: 12356131 Total methylated C's in CHG context: 405585 Total methylated C's in CHH context: 4601291 Total methylated C's in Unknown context: 2308 Total unmethylated C's in CpG context: 1987938 Total unmethylated C's in CHG context: 17770448 Total unmethylated C's in CHH context: 51290790 Total unmethylated C's in Unknown context: 16328 C methylated in CpG context: 86.1% C methylated in CHG context: 2.2% C methylated in CHH context: 8.2% C methylated in Unknown context (CN or CHN): 12.4% Bismark completed in 10d 12h 38m 11s