Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_5_s456_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 47401736 Number of alignments with a unique best hit from the different alignments: 16402431 Mapping efficiency: 34.6% Sequences with no alignments under any condition: 12742540 Sequences did not map uniquely: 18256765 Sequences which were discarded because genomic sequence could not be extracted: 30092 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3709999 ((converted) top strand) CT/GA: 3688046 ((converted) bottom strand) GA/CT: 4511078 (complementary to (converted) top strand) GA/GA: 4463216 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 96614715 Total methylated C's in CpG context: 10467052 Total methylated C's in CHG context: 400612 Total methylated C's in CHH context: 5387299 Total methylated C's in Unknown context: 2284 Total unmethylated C's in CpG context: 3184135 Total unmethylated C's in CHG context: 19480472 Total unmethylated C's in CHH context: 57695145 Total unmethylated C's in Unknown context: 16555 C methylated in CpG context: 76.7% C methylated in CHG context: 2.0% C methylated in CHH context: 8.5% C methylated in Unknown context (CN or CHN): 12.1% Bismark completed in 11d 2h 44m 35s